We sequenced the mitochondrial genome of the Western inexperienced lizard (Lacerta bilineata) utilizing Illumina know-how and extra Sanger sequencing. The assembled 17 086 bp mitogenome had a GC content material of 40.32% and consisted of 13 protein-coding genes, 22 tRNA genes, two rRNA genes, and one management area (CR), with a gene order similar to the chordate consensus. As well as, we re-sequenced the mitogenome of the intently associated Jap inexperienced lizard L. viridis utilizing the identical methods as for L. bilineata.
The mitogenomes of L. bilineata and L. viridis confirmed a sequence identification of 94.4% and 99.9%, respectively, relative to the beforehand revealed L. viridis mitogenome. The phylogenetic reconstruction primarily based on 17 Lacertinae mitogenomes utilizing Anolis carolinensis because the outgroup supported L. bilineata and its sister species L. viridis as distinct lineages. On this examine, the expression of genes coding for α-, β- and γ-syns was analyzed within the inexperienced lizard Anolis carolinensis by semiquantitative RT-PCR and Western blot.
The synuclein (syn) household includes three proteins: α-, β- and γ-syns. In people, they’re concerned in neurodegenerative ailments equivalent to Parkinson’s illness and in tumors. Members of the syn household had been sequenced in consultant species of all vertebrates and the comparative evaluation of amino acid sequences means that syns are evolutionarily conserved, however details about their expression in vertebrate lineages remains to be scarce and fully missing in reptiles. Outcomes show good expression ranges of the three syns within the lizard nervous system, equally to human syns. This, along with the excessive identification between lizard and human syns, means that these proteins fulfill evolutionarily conserved features.
Accumulation of transposable components in Hox gene clusters throughout adaptive radiation of Anolis lizards.
Transposable components (TEs) are DNA sequences that may insert elsewhere within the genome and modify genome construction and gene regulation. The function of TEs in evolution is contentious. One speculation posits that TE exercise generates genomic incompatibilities that may trigger reproductive isolation between incipient species. This predicts that TEs will accumulate throughout speciation occasions. Right here, I examined the prediction that extant lineages with a comparatively excessive price of speciation have a excessive variety of TEs of their genomes.
I sequenced and analysed the TE content material of a marker genomic area (Hox clusters) in Anolis lizards, a basic case of an adaptive radiation. Not like different vertebrates, together with intently associated lizards, Anolis lizards have excessive numbers of TEs of their Hox clusters, genomic areas that regulate growth of the morphological variations that characterize habitat specialists in these lizards. Following a burst of TE exercise within the lineage resulting in extant Anolis, TEs have continued to build up throughout or after speciation occasions, leading to a constructive relationship between TE density and lineage speciation price. These outcomes are according to the prediction that TE exercise contributes to adaptive radiation by selling speciation.
Though there was no proof that TE density per se is related to ecological morphology, the exercise of TEs in Hox clusters may have been a wealthy supply for phenotypic variation which will have facilitated the fast parallel morphological adaptation to microhabitats seen in extant Anolis lizards. The MALAT1 (Metastasis-Related Lung Adenocarcinoma Transcript 1) gene encodes a noncoding RNA that’s processed into a protracted nuclear retained transcript (MALAT1) and a small cytoplasmic tRNA-like transcript (mascRNA).
Utilizing an RNA sequence– and structure-based covariance mannequin, we recognized greater than 130 genomic loci in vertebrate genomes containing the MALAT1 3′ finish triple-helix construction and its quick downstream tRNA-like construction, together with 44 within the inexperienced lizard Anolis carolinensis. Structural and computational analyses revealed a co-occurrence of parts of the three’ finish module. MALAT1-like genes in Anolis carolinensis are extremely expressed in grownup testis, thus we named them testis-abundant lengthy noncoding RNAs (tancRNAs). These findings recommend that differential regulation of microRNAs might play a task in coordinating the timing and expression of tons of of genes concerned in regeneration.
Differential expression of conserved and novel microRNAs throughout tail regeneration within the lizard Anolis carolinensis.
Lizards are evolutionarily probably the most intently associated vertebrates to people that may lose and regrow a complete appendage. Regeneration in lizards includes differential expression of tons of of genes that regulate wound therapeutic, musculoskeletal growth, hormonal response, and embryonic morphogenesis. Whereas microRNAs are in a position to regulate massive teams of genes, their function in lizard regeneration has not been investigated. MicroRNA sequencing of inexperienced anole lizard (Anolis carolinensis) regenerating tail and related tissues revealed 350 putative novel and 196 recognized microRNA precursors.
 Polyclonal Goat anti-GST α-form |
|
GST-ANTI-1 |
Detroit R&D |
50 uL |
EUR 280 |
 Polyclonal Goat anti-GST μ-form |
|
GST-ANTI-2 |
Detroit R&D |
50 uL |
EUR 280 |
 Polyclonal Goat anti-GST p-form |
|
GST-ANTI-3 |
Detroit R&D |
50 uL |
EUR 280 |
 Rabbit Anti-Goat IgG Antibody |
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20-abx400048 |
Abbexa |
-
EUR 189.00
-
EUR 112.00
-
EUR 272.00
|
|
- Shipped within 5-7 working days.
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 Goat Anti-Rabbit IgG Antibody |
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20-abx400049 |
Abbexa |
-
EUR 189.00
-
EUR 112.00
-
EUR 272.00
|
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- Shipped within 5-7 working days.
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 Rabbit anti Goat IgG |
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20-S5801R000-VO |
Fitzgerald |
10 ml |
EUR 133 |
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Description: Rabbit anti Goat IgG antibody |
Rabbit anti Goat IgG |
|
40C-CB0536 |
Fitzgerald |
1.5 mg |
EUR 269 |
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Description: Rabbit anti Goat IgG secondary antibody |
 Goat anti Rabbit IgG |
|
40C-CB1157 |
Fitzgerald |
5 mg |
EUR 311 |
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Description: Goat anti Rabbit IgG secondary antibody |
Goat anti Rabbit IgG |
|
41-GR30 |
Fitzgerald |
10 mg |
EUR 277 |
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Description: Goat anti Rabbit IgG secondary antibody |
Goat anti Rabbit IgG |
|
41-GR31 |
Fitzgerald |
5 mg |
EUR 162 |
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Description: Goat anti Rabbit IgG secondary antibody |
Rabbit anti Goat IgG |
|
41C-CB0529 |
Fitzgerald |
2 mg |
EUR 244 |
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Description: Rabbit anti Goat IgG secondary antibody |
Goat anti Rabbit IgG |
|
41C-CB1143 |
Fitzgerald |
2 mg |
EUR 278 |
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Description: Goat anti Rabbit IgG secondary antibody |
Goat anti Rabbit IgG |
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41C-CB1150 |
Fitzgerald |
10 mg |
EUR 388 |
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Description: Goat anti Rabbit IgG secondary antibody |
Goat anti Rabbit IgG |
|
41R-1079 |
Fitzgerald |
2 mg |
EUR 149 |
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Description: Goat anti Rabbit IgG secondary antibody |
Goat anti Rabbit IgG |
|
40-GR30 |
Fitzgerald |
100 ml |
EUR 241 |
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Description: Goat anti Rabbit IgG secondary antibody |
Goat anti Rabbit IgG |
|
40-GR30S |
Fitzgerald |
100 ml |
EUR 133 |
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Description: Goat anti Rabbit IgG secondary antibody |
Rabbit anti Goat IgG |
|
E61I01002 |
EnoGene |
1mg |
EUR 343 |
 goat Anti Rabbit IgG |
|
E61I01301 |
EnoGene |
1mg |
EUR 274 |
 Antibody) Goat Anti-Rabbit IgG (Fc) Antibody |
|
abx023409-10mg |
Abbexa |
10 mg |
EUR 857 |
- Shipped within 5-10 working days.
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Goat Anti-Rabbit IgG (Fc) Antibody |
|
abx023410-5mg |
Abbexa |
5 mg |
EUR 495 |
- Shipped within 5-10 working days.
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Goat Anti-Rabbit IgG (Fc) Antibody |
|
abx023411-2mg |
Abbexa |
2 mg |
EUR 370 |
- Shipped within 5-10 working days.
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 Antibody) Goat Anti-Rabbit IgG (FC) Antibody |
|
abx024171-2ml |
Abbexa |
2 ml |
EUR 606 |
- Shipped within 5-10 working days.
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) Goat Anti-Rabbit IgG Antibody (TRITC) |
|
abx019224-01ml |
Abbexa |
0.1 ml |
EUR 244 |
- Shipped within 5-10 working days.
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) Rabbit Anti-Goat IgG Antibody (Biotin) |
|
abx117256-500ug |
Abbexa |
500 ug |
EUR 300 |
- Shipped within 5-10 working days.
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) Rabbit Anti-Goat IgG Antibody (FITC) |
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abx117267-500ug |
Abbexa |
500 ug |
EUR 300 |
- Shipped within 5-10 working days.
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) Rabbit Anti-Goat IgG Antibody (HRP) |
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abx117278-500ug |
Abbexa |
500 ug |
EUR 300 |
- Shipped within 5-10 working days.
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) Rabbit Anti-Goat IgG Antibody (Unconjugated) |
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abx117289-500ug |
Abbexa |
500 ug |
EUR 300 |
- Shipped within 5-10 working days.
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Rabbit Anti-Goat IgG Antibody (Biotin) |
|
20-abx400010 |
Abbexa |
-
EUR 189.00
-
EUR 112.00
-
EUR 272.00
|
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- Shipped within 5-7 working days.
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Rabbit Anti-Goat IgG Antibody (FITC) |
|
20-abx400011 |
Abbexa |
-
EUR 189.00
-
EUR 112.00
-
EUR 272.00
|
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- Shipped within 5-7 working days.
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Rabbit Anti-Goat IgG Antibody (HRP) |
|
20-abx400012 |
Abbexa |
-
EUR 189.00
-
EUR 112.00
-
EUR 272.00
|
|
- Shipped within 5-7 working days.
|
) Goat Anti-Rabbit IgG Antibody (Biotin) |
|
20-abx400041 |
Abbexa |
-
EUR 189.00
-
EUR 112.00
-
EUR 272.00
|
|
- Shipped within 5-7 working days.
|
) Goat Anti-Rabbit IgG Antibody (FITC) |
|
20-abx400042 |
Abbexa |
-
EUR 189.00
-
EUR 112.00
-
EUR 272.00
|
|
- Shipped within 5-7 working days.
|
) Goat Anti-Rabbit IgG Antibody (HRP) |
|
20-abx400043 |
Abbexa |
-
EUR 189.00
-
EUR 112.00
-
EUR 272.00
|
|
- Shipped within 5-7 working days.
|
 Antibody) Rabbit anti-Goat IgG (Fc) Antibody |
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abx401456-1mg |
Abbexa |
1 mg |
EUR 370 |
|
|
 Antibody) Rabbit anti-Goat IgG (Fab') Antibody |
|
abx401536-05mg |
Abbexa |
0.5 mg |
EUR 370 |
|
|
 Rabbit Anti-Sheep/Goat IgG antibody |
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STJ99581-100l |
St John's Laboratory |
100 µl |
EUR 87 |
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Description: Unconjugated Rabbit monoclonal to Sheep/Goat IgG antibody |
Rabbit Anti-Sheep/Goat IgG antibody |
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STJ99581-1mL |
St John's Laboratory |
1 mL |
EUR 455 |
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Description: Unconjugated Rabbit monoclonal to Sheep/Goat IgG antibody |
 Goat anti Rabbit IgG Fc |
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41C-CB1136 |
Fitzgerald |
2 mg |
EUR 249 |
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Description: Goat anti Rabbit IgG secondary antibody |
 Rabbit anti Goat IgG Fc |
|
41R-1013 |
Fitzgerald |
1 mg |
EUR 188 |
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Description: Rabbit anti Goat IgG Fc fragment secondary antibody |
Rabbit anti Goat IgG Fc |
|
41R-1014 |
Fitzgerald |
1 mg |
EUR 207 |
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Description: Rabbit anti Goat IgG Fc fragment secondary antibody |
) Goat anti Rabbit IgG (FITC) |
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43-GR59 |
Fitzgerald |
2 mg |
EUR 314 |
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Description: Goat anti Rabbit IgG secondary antibody (FITC) |
) Rabbit anti Goat IgG (FITC) |
|
43C-CB0523 |
Fitzgerald |
2 mg |
EUR 286 |
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Description: Rabbit anti Goat IgG secondary antibody (FITC) |
) Rabbit anti Goat IgG (rhodamine) |
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43C-CB0524 |
Fitzgerald |
2 mg |
EUR 291 |
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Description: Rabbit anti Goat IgG secondary antibody (Rhodamine) |
) Rabbit anti Goat IgG (biotin) |
|
43C-CB0526 |
Fitzgerald |
2 mg |
EUR 291 |
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Description: Rabbit anti Goat IgG secondary antibody (biotin) |
) Rabbit anti Goat IgG (HRP) |
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43C-CB0527 |
Fitzgerald |
2 mg |
EUR 330 |
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Description: Rabbit anti Goat IgG secondary antibody (HRP) |
Rabbit anti Goat IgG (FITC) |
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43C-CB0530 |
Fitzgerald |
2 mg |
EUR 291 |
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Description: Rabbit anti Goat IgG secondary antibody (FITC) |
Rabbit anti Goat IgG (rhodamine) |
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43C-CB0531 |
Fitzgerald |
2 mg |
EUR 291 |
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Description: Rabbit anti Goat IgG secondary antibody (Rhodamine) |
Rabbit anti Goat IgG (biotin) |
|
43C-CB0533 |
Fitzgerald |
2 mg |
EUR 301 |
|
Description: Rabbit anti Goat IgG secondary antibody (biotin) |
Rabbit anti Goat IgG (HRP) |
|
43C-CB0534 |
Fitzgerald |
2 mg |
EUR 350 |
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Description: Rabbit anti Goat IgG secondary antibody (HRP) |
Rabbit anti Goat IgG (FITC) |
|
43C-CB0537 |
Fitzgerald |
1 mg |
EUR 311 |
|
Description: Rabbit anti Goat IgG secondary antibody (FITC) |
Rabbit anti Goat IgG (rhodamine) |
|
43C-CB0538 |
Fitzgerald |
1 mg |
EUR 284 |
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Description: Rabbit anti Goat IgG secondary antibody (Rhodamine) |
Rabbit anti Goat IgG (biotin) |
|
43C-CB0540 |
Fitzgerald |
1 mg |
EUR 377 |
|
Description: Rabbit anti Goat IgG secondary antibody (biotin) |
Rabbit anti Goat IgG (HRP) |
|
43C-CB0541 |
Fitzgerald |
1 mg |
EUR 375 |
|
Description: Rabbit anti Goat IgG secondary antibody (HRP) |
Goat anti Rabbit IgG (FITC) |
|
43C-CB1137 |
Fitzgerald |
2 mg |
EUR 274 |
|
Description: Goat anti Rabbit IgG secondary antibody (FITC) |
) Goat anti Rabbit IgG (rhodamine) |
|
43C-CB1138 |
Fitzgerald |
2 mg |
EUR 280 |
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Description: Goat anti Rabbit IgG secondary antibody (Rhodamine) |
) Goat anti Rabbit IgG (biotin) |
|
43C-CB1140 |
Fitzgerald |
2 mg |
EUR 316 |
|
Description: Goat anti Rabbit IgG secondary antibody (biotin) |
) Goat anti Rabbit IgG (HRP) |
|
43C-CB1141 |
Fitzgerald |
2 mg |
EUR 373 |
|
Description: Goat anti Rabbit IgG secondary antibody (HRP) |
Goat anti Rabbit IgG (FITC) |
|
43C-CB1144 |
Fitzgerald |
2 mg |
EUR 313 |
|
Description: Goat anti Rabbit IgG secondary antibody (FITC) |
Goat anti Rabbit IgG (rhodamine) |
|
43C-CB1145 |
Fitzgerald |
2 mg |
EUR 330 |
|
Description: Goat anti Rabbit IgG secondary antibody (Rhodamine) |
Goat anti Rabbit IgG (biotin) |
|
43C-CB1147 |
Fitzgerald |
2 mg |
EUR 395 |
|
Description: Goat anti Rabbit IgG secondary antibody (biotin) |
Goat anti Rabbit IgG (HRP) |
|
43C-CB1148 |
Fitzgerald |
2 mg |
EUR 358 |
|
Description: Goat anti Rabbit IgG secondary antibody (HRP) |
Goat anti Rabbit IgG (rhodamine) |
|
43C-CB1152 |
Fitzgerald |
2 mg |
EUR 289 |
|
Description: Goat anti Rabbit IgG secondary antibody (Rhodamine) |
Goat anti Rabbit IgG (biotin) |
|
43C-CB1154 |
Fitzgerald |
2 mg |
EUR 311 |
|
Description: Goat anti Rabbit IgG secondary antibody (biotin) |
Goat anti Rabbit IgG (HRP) |
|
43C-CB1155 |
Fitzgerald |
2 mg |
EUR 369 |
|
Description: Goat anti Rabbit IgG secondary antibody (HRP) |
Goat anti Rabbit IgG (FITC) |
|
43C-CB1158 |
Fitzgerald |
2 mg |
EUR 388 |
|
Description: Goat anti Rabbit IgG secondary antibody (FITC) |
Goat anti Rabbit IgG (rhodamine) |
|
43C-CB1159 |
Fitzgerald |
2 mg |
EUR 388 |
|
Description: Goat anti Rabbit IgG secondary antibody (Rhodamine) |
Goat anti Rabbit IgG (biotin) |
|
43C-CB1161 |
Fitzgerald |
2 mg |
EUR 399 |
|
Description: Goat anti Rabbit IgG secondary antibody (biotin) |
Goat anti Rabbit IgG (HRP) |
|
43C-CB1162 |
Fitzgerald |
2 mg |
EUR 403 |
|
Description: Goat anti Rabbit IgG secondary antibody (HRP) |
) Goat anti Rabbit IgG (biotin) |
|
43R-1493 |
Fitzgerald |
2 mg |
EUR 183 |
|
Description: Goat anti Rabbit IgG secondary antibody (biotin) |
) Goat anti Rabbit IgG (FITC) |
|
43R-1494 |
Fitzgerald |
2 mg |
EUR 194 |
|
Description: Goat anti Rabbit IgG secondary antibody (FITC) |
) Goat anti Rabbit IgG (HRP) |
|
43R-1495 |
Fitzgerald |
1 mg |
EUR 138 |
|
Description: Goat anti Rabbit IgG secondary antibody (HRP) |
Goat anti Rabbit IgG Fc |
|
70R-IG011 |
Fitzgerald |
1.5 mg |
EUR 378 |
|
Description: Goat anti Rabbit IgG Fc secondary antibody |
Goat anti Rabbit IgG Fc |
|
70R-IG017 |
Fitzgerald |
2 mg |
EUR 381 |
|
Description: Goat anti Rabbit IgG Fc secondary antibody |
 Goat anti-rabbit IgG-HRP |
|
E22NR02-1 |
EnoGene |
100ul |
EUR 343 |
 Goat Anti-Rabbit IgG/HRP |
|
E3GAR001 |
EnoGene |
200ul |
EUR 408 |
 Rabbit Anti-Goat IgG/HRPAntibody |
|
E3RAG001 |
EnoGene |
200ul |
EUR 408 |
 goat Anti Rabbit IgG-Biotin |
|
E61I01302 |
EnoGene |
1mg |
EUR 499 |
 goat Anti Rabbit IgG-HRP |
|
E61I01303 |
EnoGene |
1mg |
EUR 499 |
 goat Anti Rabbit IgG-FITC |
|
E61I01304 |
EnoGene |
1mg |
EUR 611 |
 Rabbit Anti-Sheep/Goat IgG |
|
C020231-100mg |
Unibiotest |
100mg |
EUR 1368 |
Rabbit Anti-Sheep/Goat IgG |
|
C020231-1mg |
Unibiotest |
1mg |
EUR 269 |
 488 Goat-anti-Rabbit IgG |
|
GT26001 |
Neuromics |
1 mg |
EUR 375 |
 494 Goat-anti-Rabbit IgG |
|
GT26002 |
Neuromics |
1 mg |
EUR 375 |
 546 Goat-anti-Rabbit IgG |
|
GT26004 |
Neuromics |
1 mg |
EUR 375 |
 642 Goat-anti-Rabbit IgG |
|
GT26006 |
Neuromics |
1 mg |
EUR 375 |
 Goat Anti-Rabbit IgG-HRP |
|
LF-SA8002 |
Abfrontier |
0.5 ml |
EUR 121 |
|
Description: Goat polyclonal to Rabbit IgG |
Goat Anti-Rabbit IgG-HRP |
|
LF-SA8002A |
Abfrontier |
1.0 ml (0.5 ml X 2) |
EUR 171 |
|
Description: Goat polyclonal to Rabbit IgG |
 Goat Anti-Rabbit IgG-AP |
|
LF-SA8002AP |
Abfrontier |
1.0 ml |
EUR 312 |
|
Description: Goat polyclonal to Rabbit IgG |
 Goat Anti-Rabbit IgG-BIOT |
|
LF-SA8002B |
Abfrontier |
1.0 mg |
EUR 332 |
|
Description: Goat polyclonal to Rabbit IgG |
 Goat Anti-Rabbit IgG-FITC |
|
LF-SA8002F |
Abfrontier |
1.0 mg |
EUR 261 |
|
Description: Goat polyclonal to Rabbit IgG |
 Goat Anti-Rabbit IgG-TRITC |
|
LF-SA8002TR |
Abfrontier |
1.0 mg |
EUR 248 |
|
Description: Goat polyclonal to Rabbit IgG |
 Goat Anti-Rabbit IgG-UNLB |
|
LF-SA8002U |
Abfrontier |
1.0 mg |
EUR 248 |
|
Description: Goat polyclonal to Rabbit IgG |
 Goat Anti-Rabbit IgG-PE |
|
LF-SA8060 |
Abfrontier |
1 ml |
EUR 412 |
|
Description: Goat polyclonal to Rabbit IgG |
 Goat Anti-rabbit IgG-ALP |
|
STJ15100034 |
St John's Laboratory |
1 ml |
EUR 350 |
|
Description: Goat anti-rabbit IgG-ALP is specific for rabbit IgG H- and L-chains and was purified using immunoaffinity chromatography. |
Eleven microRNAs had been differentially expressed between the regenerating tail tip and base throughout most outgrowth (25 days publish autotomy), together with miR-133a, miR-133b, and miR-206, which have been reported to manage regeneration and stem cell proliferation in different mannequin programs. Three putative novel differentially expressed microRNAs had been recognized within the regenerating tail tip. Differentially expressed microRNAs had been recognized within the regenerating lizard tail, together with recognized regulators of stem cell proliferation. The identification of three putative novel microRNAs means that regulatory networks, both conserved in vertebrates and beforehand uncharacterized or particular to lizards, are concerned in regeneration.
 Polyclonal Antibody)
 Polyclonal Antibody)